Optimizing Different Medium Component Concentration and Temperature Stress Pretreatment for Gynogenesis Induction in Unpollinated Ovule Culture of Sugar Beet (Beta vulgaris L.)
نویسندگان
چکیده
The great economic importance of sugar beet determines the ongoing biotechnological studies conducted worldwide to improve technology obtaining doubled haploids (DHs) using method unpollinated ovule culture in vitro. To induction gynogenesis, we tested effect thidiazuron (TDZ), temperature bud pretreatment, different concentrations sucrose, and culturing on liquid or solid medium. Three genotypes were this study. use TDZ at a concentration 0.4 mg/L IMB (induction medium for Beta vulgaris) nutrient with 3 g/L phytagel, 50 200 ampicillin cultivation 28◦C dark produced up 16.7% induced ovules. same composition 8% Increasing 0.8 resulted reduction total inhibition depending genotype. Reducing sucrose 20 increasing it 80 was not effective. In all three genotypes, absence pretreatment buds (5–6 °C) showed best results. plant regeneration MS 1 6-benzylaminopurine (BAP) 0.1 gibberellic acid (GA3) seven shoots from one most responsive We by flow cytometry, chromosome counting chloroplast number assessment that regenerant plants haploid (2n = x 9).
منابع مشابه
Sugar beet (Beta vulgaris L.).
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Objective: Many agronomic practices may need to be adjusted to maximize yield and quality of sugar beet. Thus agronomic package must be always modified. This sutdy was laid out for evaluation of changes in carbohydrates and sugar yield in sugar beet (Beta vulgaris L.) under different biofertilizers and irrigation closed time at Dorud region of Iran, during 2013. Methods: The experiment...
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ژورنال
عنوان ژورنال: Horticulturae
سال: 2023
ISSN: ['2311-7524']
DOI: https://doi.org/10.3390/horticulturae9080900